First Horse Sarcoid Treatment by Electrochemotherapy: Preliminary Experimental Results , by Y. Tamzali, J. Teissie and M. P. Rols; P0662.1103

Electrochemotherapy in veterinary oncology.

Electropermeabilization is a method that uses electric field pulses to induce an electrically mediated reorganization of the plasma membrane of cells. Electrochemotherapy combines local or systemic administration of chemotherapeutic drugs such as bleomycin or cisplatin that have poor membrane permeability with electropermeabilization by direct application of electric pulses to the tumors. Precl...

Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells

Electric field mediated gene transfer is facing a problem in expression yield due to the poor transfer across the nuclear envelope. Trans-cyclohexane-1,2-diol (TCHD) was shown to significantly increase chemically mediated transfection by collapsing the permeability barrier of the nuclear pore complex. We indeed observed a significant increase in expression by electrotransfer when cells are trea...

Antitumor activity of 2',3'-dideoxycytidine nucleotide analog against tumors up-regulating DNA polymerase beta.

DNA polymerase beta (Pol beta), an error-prone DNA-synthesizing enzyme tightly down-regulated in healthy somatic cells, has been shown to be overexpressed in many human tumors. In this study, we show that treatment with the 2',3'-dideoxycytidine (ddC) nucleoside analog inhibited in vitro and in vivo the proliferation of Pol beta-transfected B16 melanoma cells, which up-regulate Pol beta compare...

Experimental Transmission of African Horse-Sickness by Means of Mosquitoes

Biomedical applications of electric pulses with special emphasis on antitumor electrochemotherapy

Short and intense electric pulses (EP) are regularly used in almost all molecular and cellular biology laboratories to introduce foreign DNA, as well as other exogeneous molecules, into living cells. Besides these in vitro applications, some in vivo applications have recently emerged. Biomedical application of EP is thus a new interdisciplinary field at the frontier of physics, chemistry and bi...